Functional amplification and preservation of human gut microbiota
| Autor: | Nadia Gaci, Prem Prashant Chaudhary, William Tottey, Monique Alric, Jean-François Brugère |
|---|---|
| Přispěvatelé: | Conception, Ingénierie et Développement de l'Aliment et du Médicament (CIDAM), Université d'Auvergne - Clermont-Ferrand I (UdA), Microbiologie Environnement Digestif Santé (MEDIS), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), INRA Clermont-Ferrand-Theix-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
cryoprotective agent (CPA)
fecal microbiota transplantation (FMT) glycerol dimethylsulfoxide digestive system environmental control system for intestinal microbiota (ECSIM) [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology lcsh:Microbial ecology fluids and secretions gut microbiota preservation polyethylene glycol lcsh:QR100-130 [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition Research Article |
| Zdroj: | Microbial Ecology in Health and Disease Microbial Ecology in Health and Disease, 2017, 28 (1), pp.1308070. ⟨10.1080/16512235.2017.1308070⟩ Microbial Ecology in Health and Disease, Co-Action Publishing, 2017, 28 (1), pp.1308070. ⟨10.1080/16512235.2017.1308070⟩ Microbial Ecology in Health and Disease, Vol 28, Iss 1 (2017) |
| ISSN: | 0891-060X |
| Popis: | Background: The availability of fresh stool samples is a prerequisite in most gut microbiota functional studies. Objective: Strategies for amplification and long-term gut microbiota preservation from fecal samples would favor sample sharing, help comparisons and reproducibility over time and between laboratories, and improve the safety and ethical issues surrounding fecal microbiota transplantations. Design: Taking advantage of in vitro gut-simulating systems, we amplified the microbial repertoire of a fresh fecal sample and assessed the viability and resuscitation of microbes after preservation with some common intracellular and extracellular acting cryoprotective agents (CPAs), alone and in different combinations. Preservation efficiencies were determined after 3 and 6 months and compared with the fresh initial microbiota diversity and metabolic activity, using the chemostat-based Environmental Control System for Intestinal Microbiota (ECSIM) in vitro model of the gut environment. Microbial populations were tested for fermentation gas, short-chain fatty acids, and composition of amplified and resuscitated microbiota, encompassing methanogenic archaea. Results: Amplification of the microbial repertoire from a fresh fecal sample was achieved with high fidelity. Dimethylsulfoxide, alone or mixed with other CPAs, showed the best efficiency for functional preservation, and the duration of preservation had little effect. Conclusions: The amplification and resuscitation of fecal microbiota can be performed using specialized in vitro gut models. Correct amplification of the initial microbes should ease the sharing of clinical samples and improve the safety of fecal microbiota transplantation. Abbreviations: CDI, Clostridium difficile infection; CPA, cryoprotective agent; D, DMSO, dimethylsulfoxide; FMT, fecal microbiota transplantation; G, glycerol; IBD, inflammatory bowel disease; P, PEG-4000, polyethylene glycol 4000 g.mol−1; SCFA, short-chain fatty acid; SNR, signal-to-noise ratio |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|