Revealing impaired pathways in the an11 mutant by high-throughput characterization of petunia axillaris and petunia inflata transcriptomes
| Autor: | Zenoni, S., D'Agostino, N., Tornielli, G.B., Quattrocchio, F.M., Chiusano, M.L., Koes, R.E., Zethof, J., Guzzo, F., Delledonne, M., Frusciante, L., Gerats, T., Pezzotti, M. |
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| Přispěvatelé: | Developmental Genetics, AIMMS, Zenoni, S., D'Agostino, Nunzio, Tornielli, G. B., Quattrocchio, F., Chiusano, MARIA LUISA, Koes, R., Zethof, J., Guzzo, F., Delledonne, Massimo, Frusciante, Luigi, Gerats, T., Pezzotti, M. |
| Rok vydání: | 2011 |
| Předmět: |
Petunia axillaris
Petunia inflata next-generation sequencing transcriptome AN11 Down-Regulation Flowers Plant Genetics Gene Expression Regulation Plant Consensus Sequence Proanthocyanidins Oligonucleotide Array Sequence Analysis Plant Proteins Base Sequence Plant Extracts Gene Expression Profiling High-Throughput Nucleotide Sequencing Molecular Sequence Annotation Sequence Analysis DNA Up-Regulation Petunia RNA Plant Seedlings Mutation Seeds Transcriptome Genome Plant Transcription Factors |
| Zdroj: | The Plant Journal, 68, 1, pp. 11-27 Plant Journal, 2011(68), 11-17. Wiley-Blackwell The Plant Journal, 68, 11-27 Zenoni, S, D'Agostino, N, Tornielli, G B, Quattrocchio, F M, Chiusano, M L, Koes, R E, Zethof, J, Guzzo, F, Delledonne, M, Frusciante, L, Gerats, T & Pezzotti, M 2011, ' Revealing impaired pathways in the an11 mutant by high-throughput characterization of Petunia axillaris and Petunia inflata transpcriptomes ', Plant Journal, vol. 2011, no. 68, pp. 11-17 . https://doi.org/10.1111/j.1365-313X.2011.04661.x |
| ISSN: | 0960-7412 |
| DOI: | 10.1111/j.1365-313X.2011.04661.x |
| Popis: | Petunia is an excellent model system, especially for genetic, physiological and molecular studies. Thus far, however, genome-wide expression analysis has been applied rarely because of the lack of sequence information. We applied next-generation sequencing to generate, through de novo read assembly, a large catalogue of transcripts for Petunia axillaris and Petunia inflata. On the basis of both transcriptomes, comprehensive microarray chips for gene expression analysis were established and used for the analysis of global- and organ-specific gene expression in Petunia axillaris and Petunia inflata and to explore the molecular basis of the seed coat defects in a Petunia hybrida mutant, anthocyanin 11 (an11), lacking a WD40-repeat (WDR) transcription regulator. Among the transcripts differentially expressed in an11 seeds compared with wild type, many expected targets of AN11 were found but also several interesting new candidates that might play a role in morphogenesis of the seed coat. Our results validate the combination of next-generation sequencing with microarray analyses strategies to identify the transcriptome of two petunia species without previous knowledge of their genome, and to develop comprehensive chips as useful tools for the analysis of gene expression in P. axillaris, P. inflata and P. hybrida. © 2011 Blackwell Publishing Ltd. |
| Databáze: | OpenAIRE |
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