Separation of acidic peptides by reversed-phase ion-pair chromatography. Analytical application to a series of acidic substrates of casein kinases

Autor: Calderan, Andrea, Ruzza, Paolo, Marin, Oriano, Secchieri, M, Borin, Gianfranco, Marchiori, Fernando
Rok vydání: 1991
Předmět:
Zdroj: Journal of chromatography. 548(1-2)
Popis: A series of small peptides including clusters of glutamyl residues, synthesized to study the site specificity of rat liver (L-CK2) and yeast (Y-CK2) casein kinase-2, are analytically characterized by ion-pair high-performance liquid chromatography using tetrabutylammonium as counter-ion and acetonitrile as modifier of the aqueous phase. Under these conditions peptides of slightly different acidity can be separated and the elution order parallels the hydrophobicity of the ion-pair-peptide complexes, which increases with the number of the acidic functions present in the sequence.
Databáze: OpenAIRE