| Autor: |
Milano, F., Jorritsma, T., Rygiel, A. M., Bergman, J. J., Sondermeijer, C., ten Brinke, A., van Ham, S. M., Krishnadath, K. K. |
| Přispěvatelé: |
Amsterdam Gastroenterology Endocrinology Metabolism, Cancer Center Amsterdam, Gastroenterology and Hepatology, Center of Experimental and Molecular Medicine, Other departments, AII - Inflammatory diseases, AII - Infectious diseases, Landsteiner Laboratory |
| Jazyk: |
angličtina |
| Rok vydání: |
2008 |
| Předmět: |
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| Zdroj: |
Scandinavian journal of immunology, 68(6), 616-623. Wiley-Blackwell |
| ISSN: |
0300-9475 |
| Popis: |
Immunotherapy for solid cancers, such as oesophageal adenocarcinoma (OAC), is generally hampered by an unfavourable immunological tumour microenvironment. This prompted us to investigate the nature of the OAC environment. Biopsies of tumour and normal control tissues were collected from 17 OAC patients, and investigated using fluorescent immunohistochemistry (IHC) for the expression of cyclooxygenase-2 (COX-2), vascular endothelial growth factor (VEGF), transforming growth factor-beta, indoleamine 2-3 dioxygenase, CXCL3 and CXCR1, and for measuring a panel of cytokines by cytometric bead array (CBA), and for Granzyme B (GrB), Perforin and PI9 detection by semi-quantitative PCR (QPCR). IHC showed that expression of all the above-mentioned factors is upregulated in 80-93% of the tumours. By QPCR, the cytokine interleukin (IL)-8 was significantly upregulated in tumour samples (P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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