Structural Remodeling of the Human Colonic Mesenchyme in Inflammatory Bowel Disease
| Autor: | Kinchen, J, Chen, H, Parikh, K, Antanaviciute, A, Jagielowicz, M, Fawkner-Corbett, D, Ashley, N, Cubitt, L, Mellado-Gomez, E, Attar, M, Sharma, E, Wills, Q, Bowden, R, Richter, F, Ahern, D, Puri, K, Henault, J, Gervais, F, Koohy, H, Simmons, A |
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| Rok vydání: | 2017 |
| Předmět: |
Tumor Necrosis Factor Ligand Superfamily Member 14
Colon Wnts mesenchyme Article Thromboplastin crypt niche Mesoderm Genetic Heterogeneity Mice stratification target discovery inflammatory bowel disease Animals Homeostasis Humans Intestinal Mucosa Myofibroblasts Wnt Signaling Pathway Cell Proliferation Inflammation TNFSF14 single-cell RNA-seq Epithelial Cells Mesenchymal Stem Cells Fibroblasts stromal cell Colitis Inflammatory Bowel Diseases Intestines Mice Inbred C57BL RAW 264.7 Cells CyTOF Single-Cell Analysis SOX6 Pericytes SOXD Transcription Factors |
| Zdroj: | Cell |
| ISSN: | 1097-4172 |
| Popis: | Summary Intestinal mesenchymal cells play essential roles in epithelial homeostasis, matrix remodeling, immunity, and inflammation. But the extent of heterogeneity within the colonic mesenchyme in these processes remains unknown. Using unbiased single-cell profiling of over 16,500 colonic mesenchymal cells, we reveal four subsets of fibroblasts expressing divergent transcriptional regulators and functional pathways, in addition to pericytes and myofibroblasts. We identified a niche population located in proximity to epithelial crypts expressing SOX6, F3 (CD142), and WNT genes essential for colonic epithelial stem cell function. In colitis, we observed dysregulation of this niche and emergence of an activated mesenchymal population. This subset expressed TNF superfamily member 14 (TNFSF14), fibroblastic reticular cell-associated genes, IL-33, and Lysyl oxidases. Further, it induced factors that impaired epithelial proliferation and maturation and contributed to oxidative stress and disease severity in vivo. Our work defines how the colonic mesenchyme remodels to fuel inflammation and barrier dysfunction in IBD. Graphical Abstract Highlights • Single-cell census of the colonic mesenchyme reveals unexpected heterogeneity • Identification of the colonic crypt niche mesenchymal cell expressing SOX6 and Wnts • Definition of fundamental aspects of mesenchymal remodeling in colitis • Analysis of colitis-associated mesenchymal cells reveals pathogenicity drivers Single-cell profiling of human colonic mesenchymal cells identifies a colitis-associated population that expresses factors contributing to epithelial cell dysfunction and inflammation. |
| Databáze: | OpenAIRE |
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