| Autor: |
Daniel Podbiel, Franz Laermer, Roland Zengerle, Jochen Hoffmann |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Microsystems & Nanoengineering, Vol 6, Iss 1, Pp 1-12 (2020) |
| ISSN: |
2055-7434 |
| Popis: |
Amplifying the scale of DNA amplification An integrated microfluidic device allows researchers to simultaneously detect and quantify multiple target DNA sequences in a single sample. A technique called the polymerase chain reaction (PCR) efficiently generates many copies of a given DNA sequence, even from tiny amounts of source material. Daniel Podbiel and colleagues at Robert Bosch GmbH in Germany have now developed a sophisticated device that can simultaneously perform many PCR reactions from one sample. Within their chip, the DNA is partitioned into multiple reaction wells, each of which can contain reagents needed to PCR amplify a different gene. These reactions are then performed on the chip, generating a fluorescence signal that can be detected and measured to reveal the amount of amplified DNA. Such a device could ultimately prove useful for conducting multi-gene analysis in medical diagnostics or other applications. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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