Plasminogen activation in human acute leukaemias
| Autor: | Scherrer, Anne Paule Marie, Wohlwend, Annelise Isabelle, Kruithof, Egbert, Vassalli, Jean-Dominique, Sappino, Pascal |
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| Rok vydání: | 1999 |
| Předmět: |
ddc:616
Plasminogen Activator Inhibitor 1/metabolism Tissue Plasminogen Activator/metabolism Precursor Cell Lymphoblastic Leukemia-Lymphoma/blood RNA Messenger/metabolism Plasminogen Receptors Cell Surface Leukemia Myeloid/blood Precursor Cell Lymphoblastic Leukemia-Lymphoma Plasminogen/metabolism Urokinase-Type Plasminogen Activator Receptors Urokinase Plasminogen Activator Urokinase-Type Plasminogen Activator/metabolism Leukemia Myeloid Tissue Plasminogen Activator Plasminogen Activator Inhibitor 2/metabolism Acute Disease Plasminogen Activator Inhibitor 1 Plasminogen Activator Inhibitor 2 Tumor Cells Cultured Humans ddc:576.5 Electrophoresis Polyacrylamide Gel RNA Messenger Receptors Cell Surface/metabolism |
| Zdroj: | British Journal of Haematology, Vol. 105, No 4 (1999) pp. 920-7 |
| ISSN: | 0007-1048 |
| Popis: | Plasminogen activation is implicated in solid tumour growth, invasion and metatastic spread. However, little is known about its role in leukaemia. We investigated the production by leukaemic cells of plasminogen activators [urokinase (uPA) and tissue-type PA (tPA)], cell surface receptor for uPA (uPAR) and PA inhibitors (PAI-1 and PAI-2). Leukaemic cells from 37 patients [26 with acute myeloid leukaemia (AML) and 11 with acute lymphoid leukaemia (ALL)] were analysed for mRNA content and enzymatic activities. High levels of uPA mRNA were found in M1, M2, M3 and M4-M5 AMLs, whereas tPA mRNA was not detected in any of the analysed cases. uPAR mRNA was confined to subtypes M4-M5. PAI-1 mRNA was detected in M3 and M4-M5. PAI-2 mRNA was found predominantly in M2 and M4-M5. SDS-PAGE/zymography analyses of cell extracts and supernatants after 24 and 48 h of culture confirmed the production of active uPA by AML cells (mainly M4-M5). but not by ALL. The finding of uPA, uPAR, PAI-1 and PAI-2 synthesized by leukaemic cells suggests that plasminogen activation may contribute to the invasive behaviour of these cells, the fibrinolytic imbalance observed in leukaemic patients and the differentiation and proliferation of M4-M5 by interaction of uPA with uPAR. |
| Databáze: | OpenAIRE |
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