Tpz1TPP1 SUMOylation reveals evolutionary conservation of SUMO-dependent Stn1 telomere association
| Autor: | Garg, Mansi, Gurung, Resham L, Mansoubi, Sahar, Ahmed, Jubed O, Davé, Anoushka, Watts, Felicity Z, Bianchi, Alessandro |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Molecular Sequence Data
SUMO-1 Protein Telomere-Binding Proteins genetic processes Shelterin Complex Evolution Molecular Schizosaccharomyces Amino Acid Sequence Telomerase CST QD0415 Tpz1 Scientific Reports fungi Sumoylation Telomere Homeostasis Telomere telomeres DNA-Binding Proteins enzymes and coenzymes (carbohydrates) Stn1 SUMO Schizosaccharomyces pombe Proteins Carrier Proteins human activities Protein Binding |
| Zdroj: | EMBO Reports |
| ISSN: | 1469-3178 |
| Popis: | Elongation of the telomeric overhang by telomerase is counteracted by synthesis of the complementary strand by the CST complex, CTC1(Cdc13)/Stn1/Ten1. Interaction of budding yeast Stn1 with overhang-binding Cdc13 is increased by Cdc13\ud SUMOylation. Human and fission yeast CST instead interact with overhang-binding TPP1/POT1. We show that the fission yeast TPP1 ortholog, Tpz1, is SUMOylated. Tpz1 SUMOylation restricts telomere elongation and promotes Stn1/Ten1 telomere association,and a SUMO-Tpz1 fusion protein has increased affinity for Stn1. Our data suggest that SUMO inhibits telomerase through stimulation of Stn1/Ten1 action by Tpz1, highlighting the evolutionary conservation of the regulation of CST function by SUMOylation. |
| Databáze: | OpenAIRE |
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