Carbonyl cyanide m-chlorophenylhydrazone induced calcium signaling and activation of plasma membrane H(+)-ATPase in the yeast Saccharomyces cerevisiae
| Autor: | Pereira, MB, Fietto, LG, Cardoso, AS, França, MM, Carvalho, FM, Trópia, MJ, Castro, IM, Brandão, RL, TISI, RENATA ANITA, MARTEGANI, ENZO |
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| Přispěvatelé: | Pereira, M, Tisi, R, Fietto, L, Cardoso, A, França, M, Carvalho, F, Trópia, M, Martegani, E, Castro, I, Brandão, R |
| Rok vydání: | 2008 |
| Předmět: |
Carbonyl Cyanide m-Chlorophenyl Hydrazone
Saccharomyces cerevisiae Proteins Ionophores Cell Membrane Saccharomyces cerevisiae BIO/11 - BIOLOGIA MOLECOLARE GTP-Binding Protein alpha Subunits Proton-Translocating ATPases Cytosol depolarizing compound Plasma membrane H+-ATPase Type C Phospholipases Calcium Calcium Signaling Protein Kinase C |
| Zdroj: | FEMS yeast research. 8(4) |
| ISSN: | 1567-1356 |
| Popis: | The plasma membrane H(+)-ATPase from Saccharomyces cerevisiae is an enzyme that plays a very important role in the yeast physiology. The addition of protonophores, such as 2,4-dinitrophenol (DNP) and carbonyl cyanide m-chlorophenylhydrazone (CCCP), also triggers a clear in vivo activation of this enzyme. Here, we demonstrate that CCCP-induced activation of the plasma membrane H(+)-ATPase shares some similarities with the sugar-induced activation of the enzyme. Phospholipase C and protein kinase C activities are essential for this activation process while Gpa2p, a G protein involved in the glucose-induced activation of the ATPase, is not required. CCCP also induces a phospholipase C-dependent increase in intracellular calcium. Moreover, we show that the availability of extracellular calcium is required for CCCP stimulation of H(+)-ATPase, suggesting a possible connection between calcium signaling and activation of ATPase. |
| Databáze: | OpenAIRE |
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