Activation of the lymphotoxin beta receptor by cross-linking induces chemokine production and growth arrest in A375 melanoma cells
| Autor: | Mariapia Degli-Esposti, Davis-Smith T, Ws, Din, Pj, Smolak, Rg, Goodwin, Ca, Smith |
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| Rok vydání: | 1997 |
| Předmět: |
Adult
Lymphotoxin-beta Antibodies Monoclonal Membrane Proteins Growth Inhibitors Receptors Tumor Necrosis Factor Immunoglobulin Fc Fragments Biopolymers Cross-Linking Reagents Fetus Solubility Lymphotoxin beta Receptor Tumor Cells Cultured Humans Tissue Distribution Chemokines Lymphotoxin-alpha Melanoma |
| Zdroj: | Europe PubMed Central |
| ISSN: | 0022-1767 |
| Popis: | The lymphotoxin beta receptor (LT beta R) was originally described as a transcribed sequence encoded on human chromosome 12p, with homology to the TNF receptor family. Subsequently, a recombinant LT beta R was shown to bind LT alpha LT beta heteromeric complexes. In this study, we have shown that LT beta R is expressed in a variety of tissues and cell lines of monocytic lineage, as well as in fibroblast and human melanoma cell lines. Unlike other members of the TNF receptor family, LT beta R is not expressed by peripheral blood T cells. A chimeric fusion protein consisting of the extracellular domain of LT beta R fused to the Fc region of human IgG1 was used to develop mAbs against LT beta R. Cross-linking LT beta R on A375 melanoma cells with these Abs generated an antiproliferative signal. In addition, the IL-8 and RANTES chemokines, early indicators of inflammation, were secreted by the A375 melanoma line and the WI38VA13 fibroblast line in response to cross-linking of LT beta R. These same activities could be induced by membrane-bound and soluble LT beta and LT alpha LT beta oligomers. |
| Databáze: | OpenAIRE |
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