Macrophages and adipocytes in human obesity: adipose tissue gene expression and insulin sensitivity during calorie restriction and weight stabilization
| Autor: | Capel, Frédéric, Klimcáková, Eva, Viguerie, Nathalie, Roussel, Balbine, Vítková, Michaela, Kováciková, Michaela, Polák, Jan, Kovácová, Zuzana, Galitzky, Jean, Maoret, Jean-José, Hanácek, Jirí, Pers, Tune H, Bouloumié, Anne, Stich, Vladimir, Langin, Dominique |
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| Přispěvatelé: | Franco-Czech Laboratory for Clinical Research on Obesity, Charles University [Prague] (CU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de médecine moléculaire de Rangueil (I2MR), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées- Institut Fédératif de Recherche Bio-médicale Institution (IFR150)-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Sports Medicine, Third Faculty of Medicine Charles University, Institute for Mother and Child Care, Center for Biological Sequence Analysis [Lyngby], Danmarks Tekniske Universitet = Technical University of Denmark (DTU), Institute of Preventive Medicine, Copenhagen University Hospital, Laboratoire de Biochimie [CHU Toulouse], Institut Fédératif de Biologie (IFB), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Pôle Biologie [CHU Toulouse], Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Simon, Marie Francoise, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-IFR150-Institut National de la Santé et de la Recherche Médicale (INSERM), Technical University of Denmark [Lyngby] (DTU), Laboratoire de Biochimie [Purpan], Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Institut Fédératif de Biologie (IFB) - Hôpital Purpan, Hôpital Purpan [Toulouse], CHU Toulouse [Toulouse]-CHU Toulouse [Toulouse]-Hôpital Purpan [Toulouse], CHU Toulouse [Toulouse] |
| Rok vydání: | 2009 |
| Předmět: |
Diet
Reducing Biopsy MESH: Insulin MESH: Energy Intake MESH: Glucose Clamp Technique MESH: Biopsy MESH: Gene Expression Profiling MESH: Reverse Transcriptase Polymerase Chain Reaction MESH: C-Reactive Protein Adipocytes MESH: Obesity Humans Insulin MESH: Genetic Variation Obesity RNA Messenger MESH: Adipocytes MESH: RNA Messenger MESH: Diet Reducing MESH: Humans Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Macrophages Body Weight MESH: Macrophages Genetic Variation MESH: Body Weight [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition C-Reactive Protein Glucose Clamp Technique Original Article Energy Intake [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition Obesity Studies |
| Zdroj: | Diabetes Diabetes, 2009, 58 (7), pp.1558-67. ⟨10.2337/db09-0033⟩ Diabetes; Vol 58 Diabetes, American Diabetes Association, 2009, 58 (7), pp.1558-67. ⟨10.2337/db09-0033⟩ |
| ISSN: | 1939-327X 0012-1797 |
| DOI: | 10.2337/db09-0033⟩ |
| Popis: | International audience; OBJECTIVE: We investigated the regulation of adipose tissue gene expression during different phases of a dietary weight loss program and its relation with insulin sensitivity. RESEARCH DESIGN AND METHODS: Twenty-two obese women followed a dietary intervention program composed of an energy restriction phase with a 4-week very-low-calorie diet and a weight stabilization period composed of a 2-month low-calorie diet followed by 3-4 months of a weight maintenance diet. At each time point, a euglycemic-hyperinsulinemic clamp and subcutaneous adipose tissue biopsies were performed. Adipose tissue gene expression profiling was performed using a DNA microarray in a subgroup of eight women. RT-quantitative PCR was used for determination of mRNA levels of 31 adipose tissue macrophage markers (n = 22). RESULTS: Body weight, fat mass, and C-reactive protein level decreased and glucose disposal rate increased during the dietary intervention program. Transcriptome profiling revealed two main patterns of variations. The first involved 464 mostly adipocyte genes involved in metabolism that were downregulated during energy restriction, upregulated during weight stabilization, and unchanged during the dietary intervention. The second comprised 511 mainly macrophage genes involved in inflammatory pathways that were not changed or upregulated during energy restriction and downregulated during weight stabilization and dietary intervention. Accordingly, macrophage markers were upregulated during energy restriction and downregulated during weight stabilization and dietary intervention. The increase in glucose disposal rates in each dietary phase was associated with variation in expression of sets of 80-110 genes that differed among energy restriction, weight stabilization, and dietary intervention. CONCLUSIONS: Adipose tissue macrophages and adipocytes show distinct patterns of gene regulation and association with insulin sensitivity during the various phases of a dietary weight loss program. |
| Databáze: | OpenAIRE |
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