Stem cells and bFGF in tendon healing: Effects of lentiviral gene transfer and long-term follow-up in a rat Achilles tendon defect model
| Autor: | Kraus, T. M., Imhoff, F. B., Reinert, J., Wexel, G., Wolf, A., Hirsch, D., Hofmann, A., Stöckle, U., Buchmann, S., Tischer, T., Imhoff, A. B., Milz, S., Anton, M., Vogt, S. |
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| Jazyk: | angličtina |
| Předmět: |
Male
Wound Healing Time Factors Genetic Vectors Lentivirus Mesenchymal stem cells (MSCs) Gene Transfer Techniques Mesenchymal Stem Cells Growth factor Genetic Therapy Recovery of Function Tendon healing Mesenchymal Stem Cell Transplantation Achilles Tendon Biomechanical Phenomena Disease Models Animal bFGF Rats Inbred Lew Tendon Injuries Animals Fibroblast Growth Factor 2 Orthopedics and Sports Medicine Lentiviral vector Research Article |
| Zdroj: | BMC Musculoskeletal Disorders |
| ISSN: | 1471-2474 |
| DOI: | 10.1186/s12891-016-0999-6 |
| Popis: | Background The influence of stem cells and lentiviral expression of basic fibroblastic growth factor (bFGF) on tendon healing and remodelling was investigated in an in-vivo long-term (12 weeks) rat Achilles tendon defect model. Methods In sixty male Lewis rats, complete tendon defects (2.4 mm) were created and either left untreated (PBS) or treated by injection of stem cells lentivirally expressing the enhanced green fluorescence marker gene eGFP (MSC-LV-eGFP) or basic fibroblast growth factor bFGF (MSC-LV-bFGF). Tendons were harvested after 12 weeks and underwent biomechanical and (immuno)-histological analysis. Results After 12 weeks the mean ultimate load to failure ratio (treated side to contralateral side) in biomechanical testing reached 97 % in the bFGF-group, 103 % in the eGFP-group and 112 % in the PBS-group. Also in the stiffness testing both MSC groups did not reach the results of the PBS group. Histologically, the MSC groups did not show better results than the control group. There were clusters of ossifications found in all groups. In immunohistology, only the staining collagen-type-I was strongly increased in both MSC groups in comparison to PBS control group. However, there were no significant differences in the (immuno)-histological results between both stem cell groups. Conclusion The biomechanical and (immuno)-histological results did not show positive effects of the MSC groups on tendon remodelling in a long-term follow-up. Interestingly, in later stages stem cells had hardly any effects on biomechanical results. This study inspires a critical and reflected use of stem cells in tendon healing. |
| Databáze: | OpenAIRE |
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