Residual cell-associated unspliced HIV-1 RNA in peripheral blood of patients on potent antiretroviral therapy represents intracellular transcripts
| Autor: | Marek, Fischer, Joseph K, Wong, Doris, Russenberger, Beda, Joos, Milos, Opravil, Bernhard, Hirschel, Alexandra, Trkola, Herbert, Kuster, Rainer, Weber, Huldrych F, Günthard |
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| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
ddc:616
HIV-1/ genetics Anti-HIV Agents Anti-HIV Agents/ therapeutic use RNA Messenger/analysis virus diseases HIV Infections Leukocytes Mononuclear/ virology Cell Line Cohort Studies Genes rev Genes tat HIV-1 Leukocytes Mononuclear RNA Viral Humans RNA Messenger HIV Infections/blood/ drug therapy/virology RNA Viral/ blood |
| Zdroj: | Antiviral Therapy, Vol. 7, No 2 (2002) pp. 91-103 |
| ISSN: | 1359-6535 |
| Popis: | Unspliced HIV-RNA (HIV-UsRNA) associated with peripheral blood mononuclear cells (PBMCs) persists in patients on potent antiretroviral therapy even in the absence of detectable plasma HIV-RNA. To further characterize such residual HIV-RNA, cell-associated virion-encapsidated HIV and intracellular unspliced HIV-RNA were differentiated and monitored using a novel highly sensitive method. In addition, expression of HIV-mRNA encoding tat and rev was assessed. PBMCs of patients with unsuppressed plasma viraemia harboured an extracellular fraction of HIV-UsRNA, which correlated highly with intracellular HIV-RNA levels. Thus, extracellular PBMC-associated HIV-RNA may, to a significant extent, reflect nascent virions attached to productively infected cells. Upon treatment with potent antiretroviral therapy resulting in plasma viraemia |
| Databáze: | OpenAIRE |
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