In vitro fermentation of total mixed diets differing in concentrate proportion: relative effects of inocula and substrates

Autor: Serment, Amélie, Giger-Reverdin, Sylvie, Schmidely, Philippe, Dhumez, Ophelie, Broudiscou, Laurent, Sauvant, Daniel
Přispěvatelé: Modélisation Systémique Appliquée aux Ruminants (MoSAR), Institut National de la Recherche Agronomique (INRA)-AgroParisTech
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: Journal of the Science of Food and Agriculture
Journal of the Science of Food and Agriculture, Wiley, 2016, 96 (1), pp.160-168. ⟨10.1002/jsfa.7076⟩
ISSN: 0022-5142
1097-0010
DOI: 10.1002/jsfa.7076⟩
Popis: BACKGROUND: In vitro techniques are used to predict ruminant feedstuff values or characterise rumen fermentation. As the results are influenced by several factors, such as the relative effects of inocula and substrates, this study aimed to examine in vitro incubation of two total mixed rations (substrates) differing in their proportion of concentrate [low (L): 350 g kg(-1) vs. high (H): 700 g kg(-1)] incubated in inocula provided by goats fed either a L or a H diet. Gas production and composition in carbon dioxide (CO2), methane (CH4) and hydrogen (H-2), volatile fatty acids (VFAs), soluble carbohydrates (SCs) and ammonia (NH3) concentrations, and pH of the fermentation fluid were measured. RESULTS: In comparison with the L inoculum and L substrate, the H ones produced more CO2 and CH4 gas, which led to higher SCs and VFA concentrations, and lower acetate-to-propionate ratio and NH3 concentration, with a predominant effect of the inoculum. CONCLUSION: The effects of the inocula and of the substrates were additive using donor animals adapted to the diets
Databáze: OpenAIRE
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