[Generation of Chicken Germ-line Chimeras by Transferring PGCs and Their Identification by AFLP.]

Autor: Xiao-Fen, Hu, Bei, Xie, Rui-Song, Yu, Qi-Zhong, Huang, De-Fu, Zhang, Lu-Sheng, Huang, Zhen, Li
Rok vydání: 2005
Předmět:
Zdroj: Yi chuan = Hereditas. 27(3)
ISSN: 0253-9772
Popis: PGCs (Primordial germ cells) were isolated from the blood of 51~56 h hatching Shiqiza chicken embryos by Ficoll density gradient centrifugation. The PGCs were injected into 2.5 d hatching embryos of H breed chicken to produce germ-line chimeras. AFLP checking method was established to identify chicken germline chimeras. Eight germ-line H-S chimera embryos were identified among 20 developing H breed embryos.
Databáze: OpenAIRE