Subcellular localization and sequence of sea urchin kinesin heavy chain: evidence for its association with membranes in the mitotic apparatus and interphase cytoplasm
| Autor: | B D, Wright, J H, Henson, K P, Wedaman, P J, Willy, J N, Morand, J M, Scholey |
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| Rok vydání: | 1991 |
| Předmět: |
Adenosine Triphosphatases
Cleavage Stage Ovum Nocodazole Blotting Western Molecular Sequence Data Restriction Mapping Antibodies Monoclonal Kinesins macromolecular substances DNA Intracellular Membranes Spindle Apparatus Articles Microtubules Cell Compartmentation Tubulin Sea Urchins Animals Calsequestrin Amino Acid Sequence Cloning Molecular Interphase Peritoneal Cavity |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 0021-9525 |
| Popis: | Kinesin was previously immunolocalized to mitotic apparatuses (MAs) of early sea urchin blastomeres (Scholey, J.M., M.E. Porter, P.M. Grissom, and J.R. McIntosh. 1985. Nature [Lond.]. 318:483-486). Here we report evidence that this MA-associated motor protein is a conventional membrane-bound kinesin, rather than a kinesin-like protein. Our evidence includes the observation that the deduced amino acid sequence of this sea urchin kinesin heavy chain is characteristic of a conventional kinesin. In addition, immunolocalizations using antibodies that distinguish kinesin from kinesin-like proteins confirm that conventional kinesin is concentrated in MAs. Finally, our immunocytochemical data further suggest that conventional kinesin is associated with membranes which accumulate in MAs and interphase asters of early sea urchin embryos, and with vesicles that are distributed in the perinuclear region of coelomocytes. Thus kinesin may function as a microtubule-based vesicle motor in some MAs, as well as in the interphase cytoplasm. |
| Databáze: | OpenAIRE |
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