| Autor: |
Wen-Li, Gu, Dong-Xia, Ye, Jing-Jing, Wu |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
Shanghai kou qiang yi xue = Shanghai journal of stomatology. 24(1) |
| ISSN: |
1006-7248 |
| Popis: |
To explore the expression and clinical significance of plasma microRNA-125b (miR-125b) in patients with oral squamous cell carcinoma (OSCC).By Stem-loop RT real-time PCR based on the SYBR Green, we investigated the expression of plasma miR-125b in 85 OSCC patients and 46 healthy controls, then evaluated its diagnostic performance as OSCC biomarker. Data analysis was performed with SPSS 17.0 software package for one-way ANOVA and receiver operating characteristic (ROC) curve.Stem-loop RT-PCR could specifically amplify miR-125b in plasma. The expression of plasma miR-125b was significantly increased in patients with OSCC compared with healthy controls (P0.05). ROC results showed that miR-125b could differentiate OSCC from healthy controls(AUC=0.966). As fold change 3.46 was chosen for optimal cutoff value, the diagnostic sensitivity and specificity was 89.4% and 93.5%, respectively.Due to the satisfactory diagnostic performance, plasma miR-125b can be used as a promising biomarker in OSCC. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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