| Autor: |
Yuan, Li, Yang, Liu, Na-Ya, Wang, Da-Zhang, Yang, Qiu-Hang, Zhang, Yue, Wang, Jie, Luo |
| Rok vydání: |
2008 |
| Předmět: |
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| Zdroj: |
Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery. 42(11) |
| ISSN: |
1673-0860 |
| Popis: |
To develop a reliable, rapid assay for detecting pathogenic aspergillus species in fungal sinusitis.Thirty-seven formalin-fixed and paraffin-embedded surgical tissue specimens from patients with fungal sinusitis were used in the present study. The aspergillus specific oligonucleotide probe was designed, commercially synthesized, and digoxigenin-labeled. Twenty-three-base oligonucleotides was selected that was complementary to 18S ribosomal RNA sequences (18S-1 probe) for detecting medically important aspergillus species.In situ hybridization for aspergillus rRNA was positive in 28 cases with the 18S-1 probe. Compared with HE (21) and methenamine-silver stain (23).In situ hybridization provides rapid and accurate identification for fungal organism in tissues, and may be useful if cultures are negative or have not performed. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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