| Autor: |
B, Desoize, L, Cravero, L, Comoe, J C, Jardillier |
| Jazyk: |
francouzština |
| Rok vydání: |
1987 |
| Předmět: |
|
| Zdroj: |
Bulletin du cancer. 74(1) |
| ISSN: |
0007-4551 |
| Popis: |
Wheat Germ Agglutinin (WGA) precipitates bone and biliary isoenzymes of serum alkaline phosphatase, when liver and intestinal isoenzymes remain in the supernatant. Biliary and intestinal isoenzymes--when present--are evaluated with electrophoresis. Isoenzymes content in precipitate and supernatant is studied by electrophoresis and heat-inactivation. The results of WGA technique are compared to those obtained with heat-inactivation. Heat inactivation reproducibility is poor when compared to WGA precipitation. Differences are found when data of 61 sera are studied. Since a reference method is lacking, results are correlated with other biological parameters, specific of hepato-biliary pathology, and with clinical data, such as metastasis localisation. This study shows that lectin precipitation yields more accurate results than heat-inactivation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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