X-ray crystallographic analysis of the hydration of A- and B-form DNA at atomic resolution

Autor:	M, Egli, V, Tereshko, M, Teplova, G, Minasov, A, Joachimiak, R, Sanishvili, C M, Weeks, R, Miller, M A, Maier, H, An, P, Dan Cook, M, Manoharan
Rok vydání:	2000
Předmět:	Oligodeoxyribonucleotides Nucleic Acid Conformation Water Spermine DNA Crystallography X-Ray Phosphates
Zdroj:	Biopolymers. 48(4)
ISSN:	0006-3525
Popis:	We have determined single crystal structures of an A-DNA decamer and a B-DNA dodecamer at 0.83 and 0.95 A, respectively. The resolution of the former is the highest reported thus far for any right-handed nucleic acid duplex and the quality of the diffraction data allowed determination of the structure with direct methods. The structures reveal unprecedented details of DNA fine structure and hydration; in particular, we have reexamined the overall hydration of A- and B-form DNA, the distribution of water around phosphate groups, and features of the water structure that may underlie the B to A transition.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::ff3ae924f3d449fb3167d3cbed791bc8 https://pubmed.ncbi.nlm.nih.gov/10699842 Zobrazit plný text záznamu Plný text