| Autor: |
M M, Liubinskaia, E V, Chernokhvostova |
| Rok vydání: |
1987 |
| Předmět: |
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| Zdroj: |
Biulleten' eksperimental'noi biologii i meditsiny. 104(9) |
| ISSN: |
0365-9615 |
| Popis: |
The proposed method of "cascade" immunization for the preparation of monospecific antisera to IgA of the experimental animals (mice, rats, guinea-pigs) requires no isolation and purification of an antigen, since it is based on using precipitation lines containing IgA. The subsequent steps of the method are the following: 1) preparation of a polyspecific antiserum against one of the secretions or IgA-containing serum fraction; 2) preparation of precipitation lines using this antiserum and some other secretions; 3) immunization of other rabbits with these precipitation lines to obtain monospecific anti-IgA antiserum. The antisera obtained do not require any additional absorption except for the removal of anti-IgA antibodies. The method can be used for the preparation of monospecific antisera to any other serum or secretion proteins. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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