[Enzyme-linked immunosorbent assay for detection of antibodies to porcine reproductive and respiratory syndrome virus, by using recombinant nucleocapsid protein N]

Autor:	V S, Bogdanova, V V, Tsibezov, V V, Grabovetskiĭ, O V, Eliseeva, T V, Grebennikova, O A, Verkhovskiĭ, A D, Zabereshchnyĭ, T I, Aliper
Rok vydání:	2007
Předmět:	Antibody Specificity Swine Porcine Reproductive and Respiratory Syndrome Animals Enzyme-Linked Immunosorbent Assay Porcine respiratory and reproductive syndrome virus Serologic Tests Reagent Kits Diagnostic Nucleocapsid Proteins Antibodies Viral Sensitivity and Specificity Recombinant Proteins
Zdroj:	Voprosy virusologii. 52(2)
ISSN:	0507-4088
Popis:	Recombinant nucleocapsid (rN) protein N of porcine reproductive and respiratory syndrome virus (PRRSV) was prepared, by using the E. coli expressiom system. Insertion of a polyhistidine marker into the structure of the protein allowed the latter to be purified by metal-chelate affinity chromatography. The purity of protein was confirmed by PAAG electrophoresis and its immunospecificity was verified by immunoblotting using rN-specific monoclonal antibodies. The protein was used as an antigen to develop indirect ELISA of PRRSV antibodies. ELISA was shown to be highly sensitive and specific.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::feb641d4400a71a5dc9558c56d6b4bc1 https://pubmed.ncbi.nlm.nih.gov/17500240 Zobrazit plný text záznamu