| Autor: |
J K, Siwicki, H, Skurzak, J A, Steffen |
| Rok vydání: |
1998 |
| Předmět: |
|
| Zdroj: |
Archivum immunologiae et therapiae experimentalis. 46(1) |
| ISSN: |
0004-069X |
| Popis: |
We have reported earlier that human T lymphocytes from blood or spleens of normal donors can be propagated in long-term culture in the presence of IL-2, after stimulation either with wheat germ agglutinin (WGA) or phytohemagglutinin (PHA) and with the use of repeated restimulation with supernatants collected after 24 h of such cultures. In the present study we demonstrate that lymphoblast lines generated with this method contain predominantly the cells expressing CD3 molecule and alpha beta heterodimer of T cell receptor (TcR alpha beta). In PHA-initiated lymphoblast lines CD4-CD8+ cells prevailed, whereas in lymphoblast lines generated after WGA-stimulation various proportion of CD4+CD8-, CD+CD8- and CD4-CD8- cells were found. In some cell lines a gradual selective outgrowth of TcR alpha beta CD4+CD8- or CD4-CD8- cells was observed. These data indicate that the long-term growth potential of human normal T lymphoblasts is not restricted to a single subset and that the surface phenotypes acquired during differentiation of the cells can be retained even after more than 100 population doublings in culture. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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