| Autor: |
V S, Pokrovskiĭ, M V, Pokrovskaia, S S, Aleksandrova, R M, Andrianov, D D, Zhdanov, N M, Omel'ianiuk, E M, Treshchalina, N N, Sokolov |
| Rok vydání: |
2013 |
| Předmět: |
|
| Zdroj: |
Prikladnaia biokhimiia i mikrobiologiia. 49(1) |
| ISSN: |
0555-1099 |
| Popis: |
The physicochemical, catalytic, and antiproliferative activity of a recombinant L-asparaginase from Yersinia pseudotuberculosis (YpA) have been studied. The following results were obtained: the K(M) value for L-asparagine is 17 +/- 0.9 microM, the optimal temperature is 60 degrees C, pH is 8.0, pI is 5.4 +/- 0.3, the L-glutaminase activity is no more than 5-6% of the L-asparaginase activity, and the antiproliferative activity on the Fisher L5178y lymphadenosis cell line comprised T/C = 136% (p0.001) at a 15% recovery rate. The described characteristic allows one to regard YpA as an antitumor enzyme with biological features similar to the L-asparaginase of E. coli. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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