| Autor: |
Sahar, Ahmed, Waleed Abdelgaber, Nemr, Walaa Awad Ahmed, Mohamed, Amany Mohamed, Mohamed, Mohamed Abd El-Fatah, Mahmoud |
| Rok vydání: |
2021 |
| Zdroj: |
Veterinary world. 15(5) |
| ISSN: |
0972-8988 |
| Popis: |
Vaccines are one of the important tools for fighting diseases and limiting their spread. The development of vaccines with high efficacy against diseases is essential. Ionizing radiation is the method used for the preparation of the irradiated gammaThe irradiated vaccines were prepared in three forms at a dose of 2×10The metabolic activity tests showed that the bacterial cells exposed to gamma radiation at the lowest lethal dose have metabolic activity. The difference in the metabolic activity between preparations of the irradiated bacterial cells varied according to the cell concentration and incubation time. The highest level of metabolic activity was 8 h after incubation in the nutrient broth medium compared with 4 and 18 h. The scanning electron microscopy of irradiated bacterial cells showed a cavity at the bacterial cell center without rupture of the surrounding cell membrane compared to the non-irradiated bacterial cells. The antibody level in the groups vaccinated with the different preparations of the irradiated bacterial cells was high compared with the control and formalin-killed vaccine groups. The level of the IFNγ showed an increase after the second dose in the group vaccinated with irradiated bacterial cells without trehalose compared with the other groups. The IL4 level in the vaccinated groups with the irradiated bacterial cells without trehalose, irradiated bacterial cells with trehalose, and trehalose lyophilized irradiated bacterial cells were at a high level when compared with the formalin-killed vaccinated group and control group after the second inoculation.The irradiated |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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