| Autor: |
Nagwa, Elkhafif, Hanan, El Baz, Olfat, Hammam, Salwa, Hassan, Faten, Salah, Wafaa, Mansour, Soheir, Mansy, Hoda, Yehia, Ahmed, Zaki, Ranya, Magdy |
| Rok vydání: |
2010 |
| Předmět: |
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| Zdroj: |
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica. 119(1) |
| ISSN: |
1600-0463 |
| Popis: |
The in vivo angiogenic potential of transplanted human umbilical cord blood (UCB) CD133(+) stem cells in experimental chronic hepatic fibrosis induced by murine schistosomiasis was studied. Enriched cord blood-derived CD133(+) cells were cultured in primary medium for 3 weeks. Twenty-two weeks post-Schistosomiasis infection in mice, after reaching the chronic hepatic fibrotic stage, transplantation of stem cells was performed and mice were sacrificed 3 weeks later. Histopathology and electron microscopy showed an increase in newly formed blood vessels and a decrease in the fibrosis known for this stage of the disease. By immunohistochemical analysis the newly formed blood vessels showed positive expression of the human-specific angiogenic markers CD31, CD34 and von Willebrand factor. Few hepatocyte-like polygonal cells showed positive expression of human vascular endothelial growth factor and inducible nitric oxide synthase. The transplanted CD133(+) human stem cells primarily enhanced hepatic angiogenesis and neovascularization and contributed to repair in a paracrine manner by creating a permissive environment that enabled proliferation and survival of damaged cells rather than by direct differentiation to hepatocytes. A dual advantage of CD133(+) cell therapy in hepatic disease is suggested based on its capability of hematopoietic and endothelial differentiation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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