Comparative effects between fucoxanthinol and its precursor fucoxanthin on viability and apoptosis of breast cancer cell lines MCF-7 and MDA-MB-231
| Autor: | Arlette, Rwigemera, Jean, Mamelona, Luc J, Martin |
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| Rok vydání: | 2015 |
| Předmět: |
Cell Nucleus
Cell Survival NF-kappa B Gene Expression Antineoplastic Agents Apoptosis Breast Neoplasms SOX9 Transcription Factor Xanthophylls beta Carotene Gene Expression Regulation Neoplastic MCF-7 Cells Humans Female Drug Screening Assays Antitumor Apoptosis Regulatory Proteins Cell Proliferation |
| Zdroj: | Anticancer research. 35(1) |
| ISSN: | 1791-7530 |
| Popis: | We evaluated whether low doses of the natural carotenoid fucoxanthin and/or of its metabolite fucoxanthinol are effective against proliferation of estrogen-sensitive MCF-7 and estrogen-resistant MDA-MB-231 breast cancer cell lines.These cell lines were stimulated with 10 to 20 μM fucoxanthin and/or fucoxanthinol, followed by cell viability assays, Annexin V immunofluorescence to evaluate apoptosis, as well as mRNA and protein extractions for changes in nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) members' expressions and nuclear translocations.Fucoxanthin and fucoxanthinol reduced the viability of MCF-7 and MDA-MB-231 cells in a time-dependent manner as a result of increased apoptosis. In both cell lines, modulatory actions of fucoxanthinol on members of the NF-κB pathway were more pronounced than that of fucoxanthin.In MDA-MB-231 cells, fucoxanthinol reduced nuclear levels of NF-κB members' p65, p52 and RelB. Fucoxanthinol and fucoxanthin could be effective for the treatment and/or prevention of breast cancer. |
| Databáze: | OpenAIRE |
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