| Autor: |
W N, Kuo, U, Ganesan, D L, Walbey, D L, Davis, K, Allen, L K, McCall |
| Rok vydání: |
1993 |
| Předmět: |
|
| Zdroj: |
Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society. 3(6) |
| ISSN: |
0954-6642 |
| Popis: |
The proteolysis of the 32P-labeled holoenzyme of cyclic AMP-dependent protein kinase (A-PKII:DEAE, peak II fraction) was analysed by SDS-polyacrylamide gel electrophoresis and autoradiography. The contaminants of the A-PKII and calpain II apparently did not interfere with the accuracy of this highly sensitive analysis. Phosphorylation of calpain II by the catalytic subunit of cyclic AMP-dependent protein kinase (A-PK) greatly enhanced the proteolysis of A-PKII, whereas phosphorylation by protein kinase C (PK-C) or cyclic GMP-dependent protein kinase (G-PK) slightly altered the proteolysis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
