Cytokine production by the bladder carcinoma cell line 5637: rapid analysis of mRNA expression levels using a cDNA-PCR procedure

Autor:	J G, Kaashoek, R, Mout, J H, Falkenburg, R, Willemze, W E, Fibbe, J E, Landegent
Rok vydání:	1991
Předmět:	Urinary Bladder Neoplasms Cytokines Gene Expression Humans DNA Neoplasm Exons RNA Messenger Blotting Northern Polymerase Chain Reaction Actins Cell Line
Zdroj:	Lymphokine and cytokine research. 10(3)
ISSN:	1056-5477
Popis:	We have studied cytokine expression by the human bladder carcinoma cell line 5637 using a cDNA-PCR procedure. Transcripts for interleukin-1 alpha (IL-1 alpha), IL-1 beta, IL-6, IL-7, IL-8, granulocyte-macrophage colony-stimulating factor (GM-CSF), G-CSF, M-CSF, tumor-necrosis factor-alpha (TNF-alpha), and TNF-beta were constitutively present, whereas IL-3, IL-4, IL-5, and IL-9 mRNA sequences could not be detected. This expression pattern was not altered after 12-O-tetradecanoyl-phorbol-13-acetate (TPA) stimulation (4 and 8 h) of 5637 cells. Relative expression levels of cytokines were assessed by limiting dilution of the cDNA pool. This procedure proved to be a semiquantitative technique when compared to Northern blot analysis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::fc3758ed0420e24f1e9c3c14876f9336 https://pubmed.ncbi.nlm.nih.gov/1883917 Zobrazit plný text záznamu