| Autor: |
Gülen, Güney-Esken, Fatima, Aerts-Kaya |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 2549 |
| ISSN: |
1940-6029 |
| Popis: |
Here, we describe a protocol for reprogramming of bone marrow-derived multipotent mesenchymal stromal/stem cells to obtain induced pluripotent stem cells from patients with primary immune deficiencies using lentiviral vectors, followed by hematopoietic differentiation of the MSC-derived iPSCs. This protocol is particularly helpful in cases where it is difficult to obtain sufficient numbers of hematopoietic cells for research and can be applied to model any hematological/immunological disease. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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