In Vivo Quantitative Assessment of Myocardial Structure, Function, Perfusion and Viability Using Cardiac Micro-computed Tomography
| Autor: | Elza, van Deel, Yanto, Ridwan, J Nicole, van Vliet, Sasha, Belenkov, Jeroen, Essers |
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| Rok vydání: | 2016 |
| Předmět: |
Mice
Inbred BALB C LV function eXIA160 Myocardium Myocardial Ischemia Contrast Media perfusion imaging Heart Bioengineering Coronary Artery Disease X-Ray Microtomography ischemia MicroCT contrast agent Imaging Mice Inbred C57BL Disease Models Animal Ventricular Dysfunction Left myocardial infarction Quantum FX Animals Issue 108 mouse cardiac functional imaging cardiovascular function LAD artery occlusion |
| Zdroj: | Journal of Visualized Experiments : JoVE |
| ISSN: | 1940-087X |
| Popis: | The use of Micro-Computed Tomography (MicroCT) for in vivo studies of small animals as models of human disease has risen tremendously due to the fact that MicroCT provides quantitative high-resolution three-dimensional (3D) anatomical data non-destructively and longitudinally. Most importantly, with the development of a novel preclinical iodinated contrast agent called eXIA160, functional and metabolic assessment of the heart became possible. However, prior to the advent of commercial MicroCT scanners equipped with X-ray flat-panel detector technology and easy-to-use cardio-respiratory gating, preclinical studies of cardiovascular disease (CVD) in small animals required a MicroCT technologist with advanced skills, and thus were impractical for widespread implementation. The goal of this work is to provide a practical guide to the use of the high-speed Quantum FX MicroCT system for comprehensive determination of myocardial global and regional function along with assessment of myocardial perfusion, metabolism and viability in healthy mice and in a cardiac ischemia mouse model induced by permanent occlusion of the left anterior descending coronary artery (LAD). |
| Databáze: | OpenAIRE |
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