| Autor: |
C, Ruiz de Alegría-Puig, A, Aguirre-Quiñonero, J, Agüero-Balbín, M P, Roiz-Mesones, L, Martínez-Martínez |
| Rok vydání: |
2016 |
| Předmět: |
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| Zdroj: |
Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia. 29(5) |
| ISSN: |
1988-9518 |
| Popis: |
Rapid identification of pathogens is essential for the diagnosis of gastrointestinal infections. Matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry has shown to be effective and fast for the identification of microorganisms. The objective of this study was to evaluate the correlation between Vitek-MSTM and conventional methods for bacterial identification causing gastrointestinal infection.A total of 329 gastrointestinal pathogens were identified using Vitek-MSTM (v2 SARAMIS MS -ID, bioMérieux, Marcy-I´Étoile, France) and routine diagnostic methods simultaneously. In cases of discrepancy 16SrRNA gene sequencing was performed.The correlation between Vitek-MSTM and diagnostic methods was 100% except for Yersinia enterocolitica (94.1%), Helicobacter pylori (10%) and Aeromonas veronii (0 %).Vitek-MSTM is a quick and useful method for identification of enterophatogenic bacteria. It is necessary to improve the performance of the system for the identification of H. pylori and A. veronii. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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