Defective T cell receptor-mediated signaling and differential induction of T cell functions by murine AIDS virus superantigen
| Autor: | O, Kanagawa, M E, Wiebenga, B A, Vaupel |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Cytotoxicity
Immunologic Isoantigens Mice Inbred BALB C Hybridomas T-Lymphocytes Receptors Antigen T-Cell Lymphocyte Activation Minor Lymphocyte Stimulatory Antigens Leukemia Virus Murine Mice Inbred C57BL Mice Murine Acquired Immunodeficiency Syndrome Animals Tetradecanoylphorbol Acetate Antigens Viral |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 150(5) |
| ISSN: | 0022-1767 |
| Popis: | A B cell line, B6-1710, expressing murine AIDS virus superantigen stimulates T cell hybridomas derived from a Ld-specific CTL clone to produce IL-2 regardless of their CD4/CD8 phenotype. However, B6-1710 cell did not stimulate the original CTL clone, L3, in either proliferation or cytolytic assays. Both B6-1710 and Ld+ P815 cells stimulated a significant Ca2+ influx in the T cell hybridomas. In contrast, only P815 stimulated tyrosine phosphorylation of a 19-kDa protein in the T cell hybridoma. The addition of the nonspecific protein kinase C activator PMA restored the proliferative response of L3 cells to B6-1710. PMA did not induce the lysis of B6-1710 cells by L3. These results suggest that murine AIDS virus encoded superantigen elicits a TCR-mediated signal different from that caused by nominal Ag. This different signaling by viral superantigen may be important for the development of viral pathogenesis in vivo. |
| Databáze: | OpenAIRE |
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