Cre-mediated recombination in the pituitary gland

Autor:	L J, Cushman, H L, Burrows, A F, Seasholtz, M, Lewandoski, N, Muzyczka, S A, Camper
Rok vydání:	2000
Předmět:	Recombination Genetic Genotype Integrases Gene Expression Mice Transgenic Breeding Immunohistochemistry Mice Inbred C57BL Mice Viral Proteins Glycoprotein Hormones alpha Subunit Pituitary Gland Animals Transgenes Promoter Regions Genetic
Zdroj:	Genesis (New York, N.Y. : 2000). 28(3-4)
ISSN:	1526-954X
Popis:	Organ-specific expression of a cre recombinase transgene allows for the analysis of gene function in a particular tissue or cell type. Using a 4.6 kb promoter from the mouse glycoprotein hormone alpha-subunit (alphaGSU or Cga) gene, we have generated and characterized a line of transgenic mice that express cre recombinase in the anterior and intermediate lobes of the pituitary gland. Utilizing a cre-responsive reporter transgene, alphaGSU-cre transgene expression was detected in the pituitary primordium and in all five cell types of the adult anterior pituitary. alphaGSU-cre transgene activity was also detected in the cardiac and skeletal muscle. Little or no activity was evident in the gonads, adrenal glands, brain, ventromedial hypothalamus, or kidneys. The alphaGSU-cre transgenic mice characterized here will be a valuable tool for examining gene function in the pituitary gland.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::fbb12d427420d064ebc69377bbc3e901 https://pubmed.ncbi.nlm.nih.gov/11105060 Zobrazit plný text záznamu Plný text