| Autor: |
Ya-Ting, Zhang, Zhao-Fan, Luo, Jian-Pei, Fang, Hai-Xia, Guo, Ke, Huang, Chi-Kong, Li |
| Rok vydání: |
2010 |
| Předmět: |
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| Zdroj: |
Zhongguo shi yan xue ye xue za zhi. 18(5) |
| ISSN: |
1009-2137 |
| Popis: |
This study was purposed to detect the minimal residual disease (MRD) in childhood acute lymphoblastic leukemia (ALL) by using real time quantitative PCR (RQ-PCR) . The Ig and TCR gene rearrangements were amplified by using 18 primer sets in B-ALL, 8 primer sets in T-ALL; the ALL-MRD levels were quantified by using RQ-PCR with SYBR green dye staining and clone specific Ig/TCR gene rearrangements as molecular markers. The results indicated that there were 8 cases showing gene rearrangements in 9 B-ALL patients, marker detection rate for all samples was 88.8%, the MRD level on day 33 during induction treatment decreased significantly. It is concluded that Ig/TCR gene rearrangements can be used as a marker to detect MRD in childhood ALL; the technique of QR-PCR with SYBR green dye staining is reliable, relatively sensitive and easy performable method which can be used in routine detection for childhood ALL. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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