[The use of proteases for enhancing the sensitivity of detecting ABO system antigens by an absorption-elution reaction in blood stains]

Autor: R S, Sakharov, M V, Fedulova, N B, Gureeva, Kh K, Nofal', A F, Druz', Iu P, Shupik
Rok vydání: 1998
Předmět:
Zdroj: Sudebno-meditsinskaia ekspertiza. 41(2)
ISSN: 0039-4521
Popis: The sensitivity of the absorption-elution test (AET) for the detection of A, B, and H antigens in blood traces can be appreciably increased by using serologically active proteases at different stages of the test. A sensitive AET modification is developed, detecting group antigens in far lesser amounts of the material than by routine AET without proteases. A higher sensitivity of the antigen-antibody reaction between enzyme-treated erythrocytes and tested blood traces permits the use of standard group-specific sera less active than usually; the actually solves the problem of deficit of the sera fit for this test.
Databáze: OpenAIRE