Temporal expression of platelet-derived growth factor (PDGF)-A and its receptor in human preimplantation embryos
| Autor: | C, Osterlund, H, Wramsby, A, Pousette |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Platelet-Derived Growth Factor
Receptor Platelet-Derived Growth Factor alpha Transcription Genetic Proto-Oncogene Proteins c-sis Polymerase Chain Reaction Receptor Platelet-Derived Growth Factor beta Blastocyst Proto-Oncogene Proteins Oocytes Humans Female Receptors Platelet-Derived Growth Factor RNA Messenger DNA Primers |
| Zdroj: | Molecular human reproduction. 2(7) |
| ISSN: | 1360-9947 |
| Popis: | In order to improve assisted fertilization in humans it is important to elucidate the mechanisms of control of growth and development in the early pre-embryo. Increasing evidence shows that growth factors are of importance for such control mechanisms. As platelet-derived growth factor (PDGF) has been shown to enhance growth in a number of tissues, it may also be important in human pre-embryo development. PDGF acts as a dimer (AA, BB or AB) through its receptors: alpha alpha, beta beta and alpha beta. In order to study the role of PDGF and its receptors, we have used reverse transcription-polymerase chain reaction (RT-PCR) to examine the presence of transcripts in human pre-embryos that were surplus from the in-vitro fertilization treatment of infertile couples. Transcripts for PDGF A were present in the oocyte, 8-cell, morula and blastocyst stages but not in the 4-cell stage. Transcripts for PDGF B were not detected at any stage. PDGF receptor (PDGFR)-alpha transcripts were found in the 4-cell, 8-cell and blastocyst stages but not in the oocyte or morula stages. Transcripts for PDGFR-beta were detected from the 8-cell, morula and blastocyst stages but not in the oocyte or 4-cell stages. These results show that mRNA synthesis of both PDGF A and the two receptor subunits alpha and beta takes place from the 8-cell stage onwards, suggesting an autostimulatory pathway as a possible mechanism for growth factors during pre-embryo development. |
| Databáze: | OpenAIRE |
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