| Autor: |
Ren, Jimin, Shang, Ty, Sherry, A. Dean, Malloy, Craig R. |
| Jazyk: |
angličtina |
| Rok vydání: |
2018 |
| Předmět: |
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| Popis: |
PURPOSE: The study was undertaken to demonstrate that there are more than one component in the extracellular inorganic phosphate (31)P signal (Pi(ex)) acquired from human head using non-localized (31)P MRS. METHODS: Outer-volume-suppression (OVS) saturation and 1D/2D (31)P chemical shift imaging (CSI) were utilized to reveal the presence of an additional component in the Pi(ex) signal. RESULTS: 67% of the head extracellular Pi signal was attenuated upon OVS saturation of the peripheral meningeal tissues, likely reflecting elimination of the Pi signal in the meningeal fluids (the blood and CSF). Localized 1D/2D CSI data provided further support for this assignment. Upon correction for the meningeal contribution, the extracellular Pi concentration was 0.51 ± 0.07 mM, while the intracellular Pi was 0.85 ± 0.10 mM. The extracellular pH was measured as 7.32 ± 0.04 when using OVS, as compared to 7.39 ± 0.03 when measured without OVS (N = 7 subjects). CONCLUSIONS: The extracellular Pi signal acquired from the human head using non-localized (31)P MRS contains a significant component likely contributed by peripheral blood and CSF in meninges that must be removed in order to use this signal as an endogenous probe for measuring extracellular pH and other properties in the brain. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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