Heat denaturation increases the sensitivity of the cytomegalovirus loop-mediated isothermal amplification method

Autor:	Ryota, Suzuki, Masaru, Ihira, Yoshihiko, Enomoto, Hiroaki, Yano, Fumio, Maruyama, Nobuhiko, Emi, Yoshizo, Asano, Tetsushi, Yoshikawa
Rok vydání:	2010
Předmět:	Hot Temperature Cytomegalovirus Infections DNA Viral Cytomegalovirus Humans Nucleic Acid Amplification Techniques Sensitivity and Specificity
Zdroj:	Microbiology and immunology. 54(8)
ISSN:	0385-5600
Popis:	A new protocol for CMV LAMP with an additional heat denaturation step was developed. While the sensitivity of the original CMV LAMP method was 500 copies/tube, sensitivity was increased by up to 100 copies/tube by additional heat denaturation. CMV DNA was detected in 103 of 350 samples (29.4%) by the original CMV LAMP procedure and 148 of 350 samples (42.3%) by the new CMV LAMP protocol. When the pp65 antigenemia assay was used as the standard method, the sensitivity, specificity, PPV, and NPV of the new protocol were 92.9%, 77.7%, 62.2%, and 96.5%, respectively.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::f9719bba3cd01b75755171705ad26d7a https://pubmed.ncbi.nlm.nih.gov/20646211 Zobrazit plný text záznamu Plný text