| Autor: |
J M, Guilian, N, Limozin, B, Mallet, J, Di Costanzo, M, Charrel |
| Jazyk: |
francouzština |
| Rok vydání: |
1977 |
| Předmět: |
|
| Zdroj: |
Biochimie. 59(3) |
| ISSN: |
0300-9084 |
| Popis: |
The two major forms of bovine erythrocyte carbonic anhydrase have been designated as CI and CII because their high activity of the C type. Separation of both forms and isolation of CI from the ethanol chloroform extract of the hemolysate were obtained by either column chromatography on DEAE-cellulose DE 23 or on DEAE-sephadex A-50. But pure preparations of the CII form were only obtained from DEAE-sephadex A-50 which separated CII from a minor component CIv1. Comparative studies of the CI and CII forms and of the minor component CIv1 strongly suggest that CIv1 is a conformational variant of CI and CII are genetic variants differing at least in their primary structure by one Arg yields Gln substitution 56 residues from the N-acetylated terminus. Based on the large variability of the proportion of the two isozymes in heterozygous individuals, the modality of the inheritance of these enzymes is discussed. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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