| Autor: |
Yu, Yuan, Qian, Zhang, Pei-Yong, Huang, Yi-Fei, Wang, Qing-Wei, Zhou, Ren-Bao, Gan, Zai-Ping, Li |
| Rok vydání: |
2002 |
| Zdroj: |
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica. 31(5) |
| ISSN: |
0582-9879 |
| Popis: |
By exchanging the N domain and C domain of hEGF and hTGF-alpha genes by PCR, two chimeras E-TGF(EGF(1-32)-TGF-alpha(34-50))and T-EGF(TGF-alpha(1-33)-EGF(33-53))were constructed. The wild and chimeric molecules were expressed in E.coli under phoA system. The expressed hEGF, hTGF-alpha and two chimeras were purified. The EGF receptor competitive binding affinity of the four molecules was hEGFhTGF-alpha and E-TGFT-EGF and the cell proliferation stimulating activity of them was hTGF-alpha and E-TGFT-EGFhEGF. The result suggests that the N domain of hEGF and hTGF-alpha may play a major role in receptor binding activity and C domain of them may be responsible for stimulating cell proliferation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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