| Autor: |
M, Sláviková, P, Kocáková, M, Slovák, I, Vancová, V, Hajnická, J, Gasperík, N, Fuchsberger, P A, Nuttall |
| Rok vydání: |
2002 |
| Předmět: |
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| Zdroj: |
Acta virologica. 46(2) |
| ISSN: |
0001-723X |
| Popis: |
A salivary gland extract (SGE) prepared from 5-days-fed Dermacentor reticulatus female ticks was fractionated by fast protein liquid chromatography (FPLC). The effect of three FPLC fractions selected on the basis of anti-interleukin 8 (anti-IL-8) activity on vesicular stomatitis virus (VSV) nucleocapsid (N) protein formation in mouse L-cells was determined. Infected 14C-labeled cells treated with the FPLC fractions were analyzed by two-dimensional (2D) electrophoresis. The yields of VSV N protein were evaluated by Imagemaster software analysis. Most noticeable was an increase in the N protein production after treatment with the fraction 39 corresponding to the major peak of the anti-IL-8 activity. The nature of the substance in SGE that was responsible for this effect remains unclear. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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