| Autor: |
Jia-Wen, Chen, Wan-Jun, Liu, Dong-Xiong, Hu, Xiang, Wang, Srinivasan, Balamurugan, Adili, Alimujiang, Wei-Dong, Yang, Jie-Sheng, Liu, Hong-Ye, Li |
| Rok vydání: |
2016 |
| Předmět: |
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| Zdroj: |
Biotechnology and applied biochemistry. 64(5) |
| ISSN: |
1470-8744 |
| Popis: |
Oleaginous microalgae hold great promises for biofuel production. However, commercialization of microalgal biofuels remains impracticable due to the lack of suitable industrial strains with high growth rate and lipid productivity. Engineering of metabolic pathways is a potential strategy for the improvement of microalgal strains for the production of lipids and also value-added products in microalgae. Malonyl CoA-acyl carrier protein transacylase (MCAT) has been reported to be involved in fatty acid biosynthesis. Here, we identified a putative MCAT in the oleaginous marine microalga Nannochloropsis oceanica. NoMCAT overexpressing N. oceanica showed a higher growth rate and photosynthetic efficiency. The neutral lipid content of engineered lines showed a significant increase by up to 31% compared to wild type. Gas chromatography-mass spectrometry analysis revealed that NoMCAT overexpression significantly altered the fatty acid composition. The composition of eicosapentaenoic acid (C20:5), which is a polyunsaturated fatty acid necessary for animal nutrition, increased by 8%. These results demonstrate the role of MCAT in enhancing fatty acid biosynthesis and growth in microalgae, and also provide an insight into metabolic engineering of microalgae with high industrial potential. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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