Changes in membrane gangliosides: differentiation of human and murine monocytic cells

Autor:	Ryan, J. L., Yohe, H. C., Malech, H. L.
Jazyk:	angličtina
Rok vydání:	1985
Předmět:	Leukemia Myeloid Acute Mice Mice Inbred C3H Gangliosides Macrophages Cell Membrane Animals Humans Cell Differentiation Female Chromatography Thin Layer Research Article Cell Line
Zdroj:	The Yale Journal of Biology and Medicine
ISSN:	1551-4056 0044-0086
Popis:	Membrane ganglioside changes in murine peritoneal macrophages and the human promyelocytic leukemia cell line HL-60 have been assessed by two-dimensional thin-layer chromatography. C3H/HeJ mice respond to protein-containing endotoxin but are hyporesponsive to protein-free endotoxin preparations. Compared to unstimulated resident cells, protein-containing endotoxin produced an alteration in the C3H/HeJ macrophage ganglioside pattern whereas protein-free endotoxin did not. In comparison, differentiation of HL-60 cells to a neutrophil-like cell by dimethylsulfoxide gave a ganglioside pattern similar to unstimulated HL-60 cells. However, differentiation of HL-60 cells by phorbol myristate acetate to macrophage-like cells results in a large increase in the monosialoganglioside GM3. The evidence presented indicates that discrete ganglioside changes occur in murine monocytes and HL-60 cells upon induction to cells with increased macrophage functions. Images FIG. 1 FIG. 2
Databáze:	OpenAIRE
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