| Autor: |
Zh Iu, Sergeeva, L M, Burova, F I, Tovkach |
| Rok vydání: |
2006 |
| Předmět: |
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| Zdroj: |
Mikrobiolohichnyi zhurnal (Kiev, Ukraine : 1993). 68(4) |
| ISSN: |
1028-0987 |
| Popis: |
It has been shown that phage P1 of Escherichia coli is able not only to lysogenize the cells of Erwinia carotovora but also to be a source of transpozon Tn9 for mutagenesis of cryptic plasmids of this important phytopathogen. Since the amount of the introduction in one of plasmids pCA25::Tn9 is 3.8 kb, at an average, it is supposed that Tn9 is inherited as a double tandem structure. A convenient method is offered for selecting bacterial clones carrying the plasmid pCA25::Tn9 which is based on counterselection of P1-lysogens at high concentrations of chloramphenicol - 100-120 microg/ml. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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