Identification of HLA-A24 restricted pre-erythrocytic stage specific T-cell epitopes using Plasmodium falciparum synthetic peptides: a preliminary study
Autor: | Pongsri, Tippawangkosol, Thitimonrat, Duangchanda, Ratawan, Ubalee, Ronnatrai, Ruengweerayut, Kenji, Hirayama, Kesara, Na-Bangchang |
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Rok vydání: | 2009 |
Předmět: |
Adult
Antigen Presentation Erythrocytes Base Sequence Genotype HLA-A Antigens Histocompatibility Testing Molecular Sequence Data Plasmodium falciparum Epitopes T-Lymphocyte HLA-A24 Antigen Thailand Interferon-gamma Case-Control Studies Prevalence Animals Humans Amino Acid Sequence Malaria Falciparum Peptides |
Zdroj: | The Southeast Asian journal of tropical medicine and public health. 40(1) |
ISSN: | 0125-1562 |
Popis: | Fourteen (9 amino acids) peptides of Plasmodium falciparum pre-erythrocytic stage antigens, namely, TRAP, CTRP, LSA-1, STARP and MSP-1, restricted to HLA-A24 and specific to T-cell response were identified. The antigen-specific IFN-gamma responses of these synthetic peptides in malaria exposed and non-malaria exposed healthy adult volunteers were detected using the ex vivo ELISPOT assay. Five peptides from TRAP and CTRP antigens significantly increased IFN-y responses of 1/9 in malaria-exposed volunteers. There is no statistically significant difference in positive T-cell response induced by any peptides in malaria exposed volunteers when evaluated as a group. The frequency of expressed HLA-A24 in malaria-exposed and non-malaria-exposed healthy adults living in northwest and central Thailand was 90% (27/30) and 100% (12/12), respectively. Although no association between positive T-cell response and HLA-A24 was found, due to the low number of positive responders achieved, one positive responder in malaria- exposed group was presented as HLA-24. |
Databáze: | OpenAIRE |
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