| Autor: |
Chun-Hong, Li, Rong, Fu, Yi-Hao, Wang, Wen-Jing, Song, Er-Bao, Ruan, Wen, Qu, Hua-Quan, Wang, Guo-Jin, Wang, Jia, Song, Xiao-Ming, Wang, Yu-Hong, Wu, Li-Min, Xing, Hong, Liu, Li-Juan, Li, Jing, Guan, Zong-Hong, Shao |
| Rok vydání: |
2014 |
| Předmět: |
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| Zdroj: |
Zhongguo shi yan xue ye xue za zhi. 22(2) |
| ISSN: |
1009-2137 |
| Popis: |
This study was aimed to investigate the expression of microRNA-21 and its correlation with PTEN in diffuse large B cell lymphoma (DLBCL) paraffin-embedded tissues, and evaluate its potential relevance with clinical characteristics. The expression levels of miR-21 in 26 primary DLBCL and 10 normal lymph node tissue specimens were examined by real-time polymerase chain reaction. The expression of PTEN was detected by immunohistochemical staining. The results indicated that the expression of miR-21 was significantly higher in tumor tissues [6.586(1.10,38.22)] than that in normal tissues [0.791 (0.35,2.87)] (P0.05). Among 26 patients with DLBCL the expression of PTEN protein was positive in 6 patients (23%), and was negative in 20 patients (77%). In patients with DLBCL, the expression level of miR-21 was negatively correlated with the level of PTEN protein. The high expression of miR-21 was positively correlated with the level of serum LDH. The expression level of miR-21 in patients with Ann Arbor III-IV stage was obviously higher than that of patients with Ann Arbor I-II stage, but did not correlate with the subtype of patients in clinic (P0.05). It is concluded that the expression of miR-21 is high in DLBCL and its overexpression may be related with poor prognosis of DLCBL. These findings suggest that PTEN is possibly one of the targets of miR-21 in DLBCL. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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