| Autor: |
K, Shigehara, N, Shijubo, M, Ohmichi, S, Kon, Y, Shibuya, R, Takahashi, S, Morita-Ichimura, T, Tatsuno, Y, Hiraga, S, Abe, N, Sato |
| Rok vydání: |
2000 |
| Předmět: |
|
| Zdroj: |
Sarcoidosis, vasculitis, and diffuse lung diseases : official journal of WASOG. 17(2) |
| ISSN: |
1124-0490 |
| Popis: |
Active sarcoidosis is considered to be a Th1 dominant condition. We examined whether Th1 cytokines are highly expressed at inflammed lesions of Japanese patients with sarcoidosis.To investigate the mRNA expression of Th1 cytokines and IL-12 in sarcoid BAL cells, we used semiquantitative reverse transcription--polymerase chain reaction method.The mRNA expressions of Th1 cytokines (IFN-gamma and IL-2) in active sarcoid BAL cells were significantly elevated as compared with those in healthy volunteers. The proportion of positive IL-4 mRNA expression in sarcoid BAL cells was not significantly higher than that in healthy volunteers. Further, there was no significant difference in IFN-gamma mRNA levels between the groups positive and negative for IL-4 mRNA expression. Although the proportion of positive expression of IL-12 mRNA in active sarcoid BAL cells was not significantly higher than that in healthy volunteers, the group positive for IL-12 mRNA expression had significantly elevated levels of IFN-gamma mRNA than did the negative group.These results may indicate that IL-12 induces IFN-gamma expression and subsequent Th1 dominant condition in Japanese patients with sarcoidosis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
