| Autor: |
K, Ohno, F, Hata, H, Nishimori, T, Yasoshima, Y, Yanai, K, Sogahata, E, Ezoe, H, Tanaka, K, Kamiguchi, H, Isomura, R, Denno, N, Sato, K, Hirata |
| Rok vydání: |
2004 |
| Předmět: |
|
| Zdroj: |
Journal of experimentalclinical cancer research : CR. 22(4) |
| ISSN: |
0392-9078 |
| Popis: |
To elucidate metastasis mechanisms, we established a Panc-1H5 subline with a highly liver metastatic cell line and a Panc-1P4a with a highly peritoneal metastatic cell line, which were sequentially selected from the parental pancreatic cancer cell line Panc-1. Using these three cell lines, we investigated several biological properties and mRNA levels of differentially-expressed genes involved in cancer metastasis with a cDNA macroarray. The tumorigenicity, motile activity, adhesive activity and cytokine production of metastatic sublines were higher than those of parental Panc-1 cells. Particularly, in Panc-1H5 cells, adhesive activity to the extracellular matrix and angiogenetic factors increased, whereas in Panc-1P4a cells, motile activity was extremely enhanced compared with Panc-1 cells. Histopathological findings for the three cell lines were the same. In cDNA macroarray analysis of Panc-1H5 cells, 11 genes were up-regulated and 20 genes were down-regulated compared with parental Panc-1 cells. In Panc-1P4a cells, 7 genes were up-regulated and 13 genes were down-regulated compared with parental Panc-1 cells. This study provides a demonstration of global gene expression analysis of pancreatic cancer cells with liver and peritoneal metastasis and these results provide new insight into the study of human pancreatic cancer metastasis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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