| Autor: |
Jeziel D, Damasceno, Luiz R O, Tosi, Renato E R S, Santos, Jeremy C, Mottram |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 1971 |
| ISSN: |
1940-6029 |
| Popis: |
Induction of gene expression is a valuable approach for functional studies since it allows for the assessment of phenotypes without the need for clonal selection. Inducible expression can find a wide range of applications, from the study of essential genes to the characterization of overexpression of genes of interest. Here, we describe a detailed protocol for the use of the DiCre-based inducible gene expression system in Leishmania parasites. This is a tightly regulated induction system that allows for time- and dose-controlled expression of gene products, as rapidly as within 12 h. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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